GSM4202303: HCI-005 AR DHT; Homo sapiens; ChIP-Seq
Sample information curated by ChIP-Atlas
Antigen
Antigen Class
TFs and others
Antigen
AR
Cell type
Cell type Class
Breast
Cell type
Breast cancer cells
NA
NA
Attributes by original data submitter
Sample
source_name
Breast cancer PDX HCI-005 tumour
tissue
PDX model HCI-005
time point
Short-term (5 days treatment)
chip antibody
AR (Abcam Ab74272)
treatment
DHT
Sequenced DNA Library
library_strategy
ChIP-Seq
library_source
GENOMIC
library_selection
ChIP
library_construction_protocol
Tumors were cut into 30µm slices on a cryostat and fixed with 2mM disuccinimidyl glutarate for 25m followed by 1%formaldehyde for 20m prior to quenching. Chromatin was prepared and subsequently sheared by the Diagenode BioRuptor Pico for 5.5 cycles (30 sec on, 30 sec off) on ice. Sheared chromatin was immunoprecipitated with 5µg AR, 5µg ER, or 2µg H3K27Ac antibody overnight, after coupling to Protein A Dynabeads (10002D). DNA was purified using phenol:chloroform:isoamyl alcohol extraction. Libraries were prepared according to manufacturer's instructions accompanying the Qiagen Ultralow Input Library Kit (Part# 180495). ChIP-Seq; Illumina NextSeq500 (High Output v2) single-end 75 bp reads.